Year:2022   Volume: 4   Issue: 3   Area:

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  3. ID: 203

A.H. AL MAMOORI, Zina MOHAMMED SARHAN

IN VITRO SELECTION BASAL MEDIUM USED FOR SEEDS GERMINATION, ‎CALLOGENESIS INDUCTION OF LOCAL VARIETIES OF VICIA FABA L

One of the most significant winter crops consumed by humans in the Middle East is ‎the faba bean (Vicia faba L.). As many other vetches and field crops. ‎ The ability of this plant to withstand such a biotic stress may be improved with the ‎help of tissue culture.‎ This investigation was carried out to create and selection basal medium used for seeds ‎germination, callogenesis induction in three Faba bean varieties, Baraqat, Iraqi and ‎Halabi, extensively grown for personal consumption in Iran, Iraq, and Syria ‎respectively.‎ First seeds were sterilized and planted on a growth-regulatory-free MS basal medium. ‎Germination rates ranked between 24.6 to 63% because of the lethal effect of ‎phenolic compounds. ‎ Five types of explants excised on the in vitro obtained seedlings were used for callus ‎initiation: stems, shoot tips, leaves, epicotyls, and parts of the roots. They were put on ‎MS basal medium that had been increased by BAP at a dosage of 0.5, 1.0, or 1.5 ‎mg/l.. Depending on the genotypes, explants, and medium, callogenesis rates ranged ‎from 33.33 to 100%. In terms of callogenesis rate and callus weight, shoot tip ‎segments grown on media with BAP at 1 and 1.5 mg/l generated the highest outcomes ‎of all three kinds. Even though it wasn't the goal of this study, the creation of somatic ‎embryos was conceivably achievable (33%) throughout this study and happened just 2 ‎months after calluses started, suggesting the embryogenic ability of such calluses for ‎the 3 kinds examined.‎

Keywords: Faba Bean, Landraces, Callogenesis

http://dx.doi.org/10.47832/2717-8234.12.49


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