Year:2024   Volume: 6   Issue: 1   Area:

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Hider M. H. AL-SHIRIFI, Abbas K. AL-MANSOORI, Hiba Jasim HAMZA, Rabab J. H AL-HASSENY, Hasan Nsaif JASIM

DETECTION OF ROTAVIRUS TYPE A BY REVERSE TRANSCRIPTASE PCR IN CHILDREN SUFFERING ‎FROM VIRAL GASTROENTERITIS IN BABYLON PROVINCE

Rotavirus type A is one of the viruses that cause viral gastroenteritis. This research was ‎done to detection Rotavirus type A in watery stool samples of children suffering from ‎viral gastroenteritis. Stool samples were collected in four months from November 2019 to ‎March 2020 from three hospitals in Babylon province of Iraq are Hashemite Hospital was ‎collected 50 stool specimens, while Al-Noor Hospital for Maternity and General Al-‎Qasim Hospital were collected 25 stool specimens. A total of 46 males and 29 females ‎were diagnosed with viral gastroenteritis. Two methods used in the present study for ‎detection Rotavirus and Rotavirus type A were rapid test and reverse transcriptase PCR . ‎The results of rapid test were indicated to 26 positive samples for rotavirus out of 75 stool ‎samples while the results of reverse transcriptase PCR for detection Rotavirus type A ‎were indicated to 6 positive samples out of the 26 stool samples‎.‎

Keywords: Rotavirus Type A, Rapid Test, RT-PCR.

http://dx.doi.org/10.47832/2717-8234.18.28


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