MUTATION OCCURRENCE IN PTGS2GENE SEQUENCE IN CERVICITIS DUE TO TOXOPLASMOSIS AND CYTOMEGALOVIRUS INFECTIONS
In this study, venous blood samples were obtained from 100 women with Cytomegalovirus infection whose ages ranged from 18 to 60 years, and 100 healthy women as a control group. The study was conducted at Al-Elwiya teaching hospital in Baghdad city during the period from 1st November 2021 to 15th June 2022. the distrbution of study groups according to age revealed a significant difference (P=0.01) in the cervicitis patient group (34.43±11.89) in comparison with the control group (39.05±13.53). The distrbution of study groups according to the residency showed that residents of rural areas were 51(51.0%) in comparison with the control group and residents of urban areas were 49(49.0%), while the healthy control group was 51(51.0%) with no significant differences (P=0.088). A highly significant differences were bserved between the mean levels of tumor marker Ca125 in patients (25.5±0.71) in comparison with the healthy controls (19.40±0.68), and between mean levels of Ca15.3 (20.80±0.74) and the healthy control group (18.34±0.77). In addition, the mean levels of CRP was (88.06±5.88) versus the controls (6.73±0.12), with a highly significant differences (P<0.001). Also, no significant differences were seen between mean levels of Ca19.9 marker (19.62±0.79) and the control group (19.21±0.71) (P= 0.7). The distribution of polymorphism according to complete blood picture was shown in table (3) .The mean lymphocyte count was 12(60%), while there was a decrease in Hb levels 3(15%), PCV value 4(20%) and Nuetrophil count 2(10%), with no significant differences. there was no mutation occured in the analysis of rs888160762 SNP of PTGS2 gene, but showed a mutation occured in the analysis of rs20417 SNP of PTGS2 gene using Sanger sequencing. The single “C” peak indicates C homozygous alleles, while the presence of “G” and “C” peaks indicates G/C heterozygous alleles.
Keywords: Mutation, PTGS2gene, Sequence, Cervicitis, Toxoplasmosis Cytomegalovirus