ISOLATION AND PURIFICATION OF BROMELAIN ENZYME FROM PINEAPPLE JUICE AND STUDY CYTOTOXIC ACTIVITY AGAINST CANCER CELLS
The work included the isolation of Bromelain enzyme from pineapple juice using different biochemical techniques which included: ammonium sulfate precipitation, diagnosis and gel filtration chromatography on Sephadex G-100. It was found that only the second peak had a high activity for Bromelain, the apparent molecular weight of the isolated Bromelain using gel filtration chromatography and SDS-PAGE was (27000) and (24000) Dalton respectively, this study was led to distinguish the cytotoxic action of purified enzyme Bromelain from pineapple juice remove on human breast cancer (MCF-7) cells in vitro contrasted with human hepatic (WRL-68) cells utilizing MTT measure. Charted 6 crystal program incorporating non-straight relapse with one-way ANOVA followed by Dennett's different comparison test to get information addressing the mean+ SD. The outcomes uncovered high poisonousness of the concentrate against MCF-7 cells with IC50 (194.5µg/ml) and showed high-decreased action toward WRL-68 cells with high IC50 esteem while the least feasibility (26.389 ±3.73022) showed up in MCF. 7 cells under treatment with 400 µg.ml-1 contrasted and high practicality in WRL-68 cells. The review reasoned that has a significant wellspring of anticancer medications with protected and particular action on disease cells in contrast with human typical cells.
Keywords: Pineapple Plant, Gel Filtration, Breast Cell Cancer Line, Enzyme Activity Test On Cancer Cells, Liver Cell Line