Year:2022   Volume: 4   Issue: 2   Area: Mechanical Engineering

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Waleed Khalid AHMED, Fatih KÖKSAL, Abdullah Abdulkreem HASSAN, Melda MERAL OCAL, Saeed Ullah JAN

ISOLATION AND MOLECULAR DIAGNOSIS OF TRICHODERMA SPP. AND EVALUATION OF ITS EFFECTIVENESS AGAINST DERMATOPHYTES

Dermatophyte infection of the human is difficult to eradicate with drug treatment. An alternative approach to treat dermatophytes might be the application of biological control agents against fungal diseases. Trichoderma spp, were isolated from the soil in Adana province of Turkey. 4 species were identified based on DNA-ITS and ITS4 sequence. T. viride, T. virens, T. harzianum and T. lixii. We tested Trichoderma species against Trichophyton tonsurans and Microsporum canis. All isolates of Trichoderma showed promising results in reducing the growth of pathogens under in vitro conditions, with different concentrations and dual culture assay. However, antagonistic assessment of isolates of Trichoderma against pathogens T.tonsurans and M.canis by concentrations assay (5,10 and 15 ml/L) showed variation in inhibition of pathogens. The colony diameter growth values ranging in all concentrations and isolates from 0.46 to 5.40 cm, The best antifungal concentration was (15 ml/L) with T. harzianum isolate in inhibition for T. tonsurans and M. canis, which reached 0.50 and 0.46 cm respectively. For dual culture assay showed that all isolates of Trichoderma reduced growth T.tonsurans and M.canis. Moreover, the highest inhibition with T. harzianum isolate Which colony diameter reached 1.34 and 1.50 cm, respectively.

Keywords: Trichderma Spp, Molecular Diagnosis, Dermatophytes

http://dx.doi.org/10.47832/2717-8234.11.7


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