Year:2022   Volume: 4   Issue: 3   Area:

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  3. ID: 184

Zahraa Hussein KADHIM

EFFECT OF FLAVONOIDS ON THICKNESS AND DIAMETER OF TESTICULAR SEMINIFEROUS TUBULES IN ADULT MALES WISTAR RATES EXPOSED TO OXIDATIVE STRESS BY LEAD ACETATE

TThe current study aims to determination the effect of flavonoids on thickness and diameter of testicular seminiferous tubules in adult males wistar rats exposed to lead acetate which cause oxidative stress. 40 male wistar rats, each 200±10g on average, were randomly devided to four equal groups and received the following treated for 60 days: First group (C) received nothing except water as a control. Quercetin at dose (300 mg/kg/B.w.) was administered to the second group (T1). Lead acetate at dose (10 mg/kg/B.w.) was administered to the third group (T2). The fourth group received quercetin (300 mg/kg/B.w) for 30 days after receiving lead acetate (10mg/kg/B.w) for 30 days. All of the animals were sacrificed at the end of experiment. Samples of testis, epididymis and vas difference were taken for histopathological study. A significant difference (p≤0.05) in the seminiferous tubule thickness and leydig cell count was reported in a histological examination. sertoli cells, primary and secondary spermatocytes in T1 group in compared with C group. Whereas there were no significant difference in seminiferous tubules diameter in T1 group in compared with the C group. Additionally, there was a significant difference (p≤0.05) between the T2 group and the C group in terms of the thickness of the seminiferous tubules, leydig's cells and sertoli cells number, primary and secondary spermatocytes, and the diameter of the seminiferous tubules. Additionally, there was a significant difference between the T3 group and the T2 group as evidenced by an increase in seminiferous tubule thickness, leydig's cells, sertoli cells, primary and secondary spermatocytes, and a decrease in seminiferous tubule diameter. Also histological sections in control group revealed a complete spermatogenesis, normal testicular tissue architecture with normal seminiferous tubules, epididymis and vas deference tissues. In T1 group there was complete spermatogenesis, the epididymis duct filled with the sperm and extended, vas deference has normal smooth muscles fibers and sperm filled the lumen of it. In T2 group there was vaculation of spermatogonia and few number of sperms, sertoli and leydige cells, the epididymis duct empty with degeneration and destruction of stereocilia, the vas deference empty from sperm and there was mild destruction in the stereocilia and sloughing the epithelial layer of it. In T3 there was large number of spermatogonia, primary and secondary spermatocytes with spermatids, the epididymial duct filled with the sperm .Vas deference has normal smooth muscles and the sperm filled the lumen of it

Keywords: Flavonoids, Oxidative Stress, Wistar Rats, Lead Acetate, Testicular Histopathology

http://dx.doi.org/10.47832/2717-8234.12.30


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