APPLICATIONS OF CURCUMIN EXTRACT FORMULATIONS FOR THE HEALING EFFICACY ON MICE WOUNDS
This study aimed to evaluate the antibacterial activity of various formulations of alcoholic extracts of Curcuma longa (Curcumin) on Staphylococcus aureus and E. coli in wounds injured mice compared to the action of the extracts with clotrimazole antibiotic. Using the well agar diffusion technique, in vitro bactericidal activity of curcumin alcoholic extract was established against several pathogens. Curcumin extract and clotrimazole ointment was also made from a combination of extract and antibiotics to examine how different formulas affected injured skin in mice. 25 albino mice were divided into five groups and had their skin incised and treated in various ways. In vitro experiments against E. coli and Staphylococcus aureus revealed excellent potency through the zone of inhibition. Furthermore, in vivo findings of the groups given therapeutic doses of extracts revealed significant values in treatment and a reduction in clinical signs, as well as notable, exceeds of the results of the extract oily ointment at (2.5 % w\v) compared to (5 % w\w), as well as similar results between curcumin extract oily ointment (2.5 %) and curcumin extract and clotrimazole oily ointment (2.5 %) groups, at significant differences. Histopathological investigations of the groups (G2, G3, G4, and G5) that had outstanding wound healing with normal skin in varying degrees compared to the control group supported these findings (G1). Curcumin alone or combined with clotrimazole as oily formula at concentration (2.5%) show augmented wound healing efficacy compared to formula at concentration (5%) after 15days of treatment, The skin section show excellent collagen deposition with enhanced re-epithelialization regeneration. at the 7 day of treated, curcumin formula at concentration (5%) exhibited lower fibroblast and fibrocystic distribution and less infiltration of cells in tissue compared to curcumin formula at concentration (2.5%) and control which show up-regulated neovascularization with highly fibroblast presence. In conclusion, the current study demonstrated the efficacy of using an alcoholic extract (Curcumin) as a special formula in combination with the antibiotic clotrimazole to show high potential uses in wound skin healing in mice; additionally, use inefficiently retarding and preventing bacterial infections such as E. coli and S. aureus.
Keywords: Curcumin Extract, Clotrimazole, Antibacterial Activity, Histopathology, Staphylococcus Aureus, E. Coli.